Does stinging nettle (Urtica dioica) have an effect on bone formation in the expanded inter-premaxillary suture?

OBJECTIVE: To determine whether systemically given stinging nettle (SN) has an effect on bone formation in response to expansion of the rat inter-premaxillary suture. MATERIALS AND METHODS: A total of 28 male Wistar albino rats were randomly divided into 4 equal groups: control (C), only expansion (OE), SN extract given only during the expansion and retention periods (SN group; a total of 17days), and SN extract given during the nursery phase before expansion (a period of 40days) and during the expansion and retention periods (N+SN group; a total of 57days). After the 5-day expansion period was completed, the rats in the OE, SN, and N+SN groups underwent 12days of mechanical retention, after which they were sacrificed, and their premaxilla were dissected and fixed. A histologic evaluation was done to determine the number of osteoblasts, osteoclasts, and capillaries, as well as the number and intensity of inflammatory cells and new bone formation. RESULTS: Statistically significant differences were found between the groups in all histologic parameters except the ratio of intensities of inflammatory cells. New bone formation and the number of capillaries were significantly higher in the SN groups than in the other groups. The statistical analysis also showed that the numbers of osteoblasts, osteoclasts, and capillaries were highest in the N+SN group. CONCLUSION: Systemic administration of SN may be effective in accelerating new bone formation and reducing inflammation in the maxillary expansion procedure. It may also be beneficial in preventing relapse after the expansion procedure.

Effect of Royal Jelly on new bone formation in rapid maxillary expansion in rats

BACKGROUND: The aim of this study was to evaluate the effects of long and short term systemic usage of royal jelly on bone formation in the expanded maxillary suture in a rat model. MATERIAL AND METHODS: Twenty eight Wistar albino rats were randomly divided into 4 equal groups: Control (C); Only Expansion (OE), Royal Jelly (RJ) group, Royal Jelly was given to rats by oral gavage only during the expansion and retention period; Royal Jelly plus Nursery (RJN) group, Royal Jelly was given to rats by oral gavage during their nursery phase of 40 days and during the retention period. After the 5 day expansion period was completed, the rats underwent 12 days of mechanical retention. All rats were sacrificed in same time. Histological examination was performed to determine the number of osteoclasts, number of osteoblasts, number of capillaries, inflammatory cell infiltration, and new bone formation. RESULTS: New bone formation, number of osteoclasts, number of osteoblasts, and the number of capillaries in the expanded maxillary sutures were higher in the RJ and RJN groups than in the other groups. Statistical analysis also demonstrated that new bone formation and the number of osteoblasts was also highest in the RJN group. CONCLUSIONS: The systemic administration of Royal Jelly in conjunction with rapid maxillary expansion may increase the quality of regenerated bone

Effect of Royal Jelly on new bone formation in rapid maxillary expansion in rats.

BACKGROUND: The aim of this study was to evaluate the effects of long and short term systemic usage of royal jelly on bone formation in the expanded maxillary suture in a rat model. MATERIAL AND METHODS: Twenty eight Wistar albino rats were randomly divided into 4 equal groups: Control (C); Only Expansion (OE), Royal Jelly (RJ) group, Royal Jelly was given to rats by oral gavage only during the expansion and retention period; Royal Jelly plus Nursery (RJN) group, Royal Jelly was given to rats by oral gavage during their nursery phase of 40 days and during the retention period. After the 5 day expansion period was completed, the rats underwent 12 days of mechanical retention. All rats were sacrificed in same time. Histological examination was performed to determine the number of osteoclasts, number of osteoblasts, number of capillaries, inflammatory cell infiltration, and new bone formation. RESULTS: New bone formation, number of osteoclasts, number of osteoblasts, and the number of capillaries in the expanded maxillary sutures were higher in the RJ and RJN groups than in the other groups. Statistical analysis also demonstrated that new bone formation and the number of osteoblasts was also highest in the RJN group. CONCLUSIONS: The systemic administration of Royal Jelly in conjunction with rapid maxillary expansion may increase the quality of regenerated bone.

Effect of femtosecond laser treatment on the shear bond strength of a metal bracket to prepared porcelain surface.

OBJECTIVE: The aim of this study was to investigate the effects of femtosecond laser treatment (Group FS) on the shear bond strength (SBS) of a metal bracket to prepared porcelain surface, and to compare it with other surface treatment techniques [50 µm Al2O3 sandblasting (Group SB), 9.6% hydrofluoric acid gel (Group HF), and neodymium-doped yttrium aluminium garnet (Nd:YAG laser) (Group NY)]. BACKGROUND DATA: Because of the increasing number of adult patients in current orthodontic practice, achieving sufficient bond strength of composite resin to porcelain restorations without bond failure during the treatment is a challenge for orthodontists. METHODS: In total, 80 glazed feldspathic porcelain samples were prepared and randomly assigned to four groups of 20. Treated surfaces were treated with a silane agent. Brackets were bonded to porcelain samples. The specimens were stored in distilled water for 24 h and then thermocycled for 500 cycles between 5° and 55°C. The SBS of the brackets was tested with a universal testing machine at a crosshead speed of 1 mm/min, until bonding failure occurred. The data were analyzed statistically using analysis of variance (ANOVA) and Tamhane multiple comparisons tests. The results of ANOVA indicated that the SBS values varied according to the surface treatment method (p<0.001). RESULTS: Results of the Tamhane post-hoc tests indicated that the bond strength in Group NY (5.11±1.53) was significantly lower than the other groups (p<0.05). There were no statistically significant differences among Groups SB (9.07±3.76), HF (9.09±3.51), and FS (11.58±4.16) (p=0.28). CONCLUSIONS: The results of this study showed that FS treatment produced high SBS of the processes assessed; therefore, it appears to be an effective method for bonding orthodontic metal brackets to prepared porcelain surfaces.

Effect of St John's wort on bone formation in the orthopaedically expanded premaxillary suture in rats: a histological study.

BACKGROUND/OBJECTIVE: The aim of this study was to investigate the effect of systemic St John's wort (Hypericum perforatum) on bone formation in the expanded premaxillary suture in rats. MATERIALS/METHODS: A total of 28 rats were randomly divided into four groups of equal numbers: control (C); only expansion (OE); St John's wort extract given only during the expansion and retention period (a total of 17 days; SJW group); and St John's wort extract given during the nursery phase before expansion (a period of 40 days), and during the expansion and retention periods (a total of 57 days; N + SJW group). After the 5 day expansion period was completed, the rats in the OE, SJW, and N + SJW groups underwent 12 days of mechanical retention, following which they were killed, and their premaxilla dissected and fixed. Histological examination was performed to determine the number of osteoclasts and capillaries, as well as the number of osteoblasts, inflammatory cell infiltration, and the amount of new bone formation. RESULTS: Statistical analysis showed that the number of osteoclasts and capillaries, and the inflammatory cell infiltration, as well as new bone formation, were higher in the SJW and N + SJW groups than in the other groups. However, statistical analysis demonstrated that among these two groups, all parameters, with the exception of the number of capillaries, were higher in the N + SJW group than the SJW group. CONCLUSIONS/IMPLICATIONS: Although more effective in long-term usage, systemic use of St John's wort hastens new bone regeneration at the premaxillary suture and may help prevent relapse after expansion.

Isolated oligodontia in monozygotic twins.

This case report defines a case of isolated oligodontia of 9 and 10 permanent teeth in 9-year-old monozygotic twin sisters and gives information about the possible genetic and environmental etiology, related dental anomalies and treatment options. The twins have a negative family history of hypodontia and oligodontia in their parents, as well as their paternal and maternal grandmothers and first cousins. No other dental anomalies could be detected in either of the twins. With the occurrence of similarly located tooth agenesis, except for one tooth, in monozygotic twins, one may consider the influence of genetic and/or environmental factors in their etiology. Hereditary relationships associated with oligodontia could help the clinicians to predict the possibility of its occurrence in other family members and in the next generations. However, clinicians should consider oligodontia when it is not hereditary.

Effects of staining solutions on the discoloration of orthodontic adhesives: an in-vitro study.

INTRODUCTION: Our aim was to evaluate the effects of staining solutions on the discoloration of orthodontic adhesives. METHODS: Six orthodontic adhesives were investigated (Transbond XT [3M Unitek, Monrovia, Calif, USA], Heliosit Orthodontic [Ivoclar Vivadent, Liectenstein], Light Bond [Reliance Orthodontic Products, Itasca, IL, USA], Bisco Ortho [Bisco, Schamburg, IL, USA], Quick Cure [Reliance Orthodontic Products, Itasca, IL, USA], and Filtek Supreme XT [3M ESPE, St Paul, Minn, USA]), and 5 beverages (tea, cola, coffee, red wine, and yogurt). Sixty specimens were prepared. Five specimens from each group were stored in each of the 5 staining solutions. The 5 remaining specimens from each group served as the controls and were stored in distilled water. The specimens were immersed in staining solutions and water at 37°C ± 1°C for 5 days. The test period was 25 days. Before and after the test period, color measurements were carried out with a spectrophotometer, and color changes (ΔE∗) were calculated. Statistical differences were evaluated by using analysis of variance (ANOVA) and the Tukey HSD tests. RESULTS: Adhesive materials, staining agents, and their interactions were found to play statistically significant roles (P < 0.001) in color changes. Among the adhesive materials, the Light Bond water control group consistently showed the lowest ΔE∗ value for all materials, and the Filtek Supreme XT group showed the highest ΔE∗ value for all materials. After the in-vitro experimental process for staining solutions and water, unsatisfactory color stability was observed for the conventional adhesive systems except for Light Bond, Transbond XT, and Bisco Ortho water control group (ΔE∗ > 3.7), respectively. CONCLUSIONS: In esthetically critical areas, discoloration of adhesive materials for fixed orthodontics can cause patient dissatisfaction. Orthodontic composites will discolor from staining beverages during their lifespan.